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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene is observed in several tumors, which ends up in chromosome instability, and thus may possibly contribute to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical purpose in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been explained. Two added splice variants had been described but thorough nucleotide sequence info is not really nevertheless available.

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Blocking peptides are peptides that bind particularly on the concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized through the antibody.

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This information outlines more probable optimization of mobile and immunoassay parameters to make certain the best possible success are...

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This information outlines even further probable optimization of mobile and immunoassay parameters to make certain the absolute best success are...

Antibodies sure to the blocking peptide no longer bind towards the epitope within the target protein. By comparing the staining from the blocked antibody vs . the antibody by itself, you can see which staining is distinct.

Goal Info Cyclin E belongs towards the highly conserved cyclin get more info family, whose users are characterised by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinct expression and degradation patterns which add on the temporal coordination of each mitotic event. Cyclin E kinds a posh with and features like a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S changeover. This protein accumulates at the G1-S stage boundary and is degraded as cells development via S section.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both of those antibodies...

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